Targeted Analysis of Polar Metabolites

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Description

A high sample throughput assay for identification and quantification of a wide range of polar metabolites ideal for large-scale studies, ionic metabolites, and low volume samples (<5 uL or < 5 mg dried weight).

Quantitative

Absolute

Platform Available

UPLC-MRM/MS

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Name	HMDB ID	Super Class	Class
(Iso)butyrylcarnitine (C4)
3-Aminoisobutyric acid (BAIBA)
3-Hydroxyanthranilic acid
4-Aminohippuric acid
5-Hydroxyindole acetic acid
Acetyl-arginine
Acetylcarnitine (C2)
Argininosuccinate
Arginosuccinate
Asparic acid
Asymmetric dimethylarginine (SDMA)
Carboxy-ibuprofen
Carnitine (C0)
Cystine (disulfide)
Cystine disulfide
Decanoylcarnitine (C10)
Dimethyl guanosine
Dimethylglycine (DMG)
Ethyl sulfate
Ethylmalonic acid/Glutaric acid
Fructose-1,6-bisphosphate (F1,6P)
Fructose-6-phosphate (F6P)
gamma-Aminobutyic acid (GABA)
Glc-Hydroxylysine
GlcGal-Hydroxylysine
Glucose-1-phosphate (G1P)
Glucuronic acid
Glycerol phosphate
Glytamyl-cysteine
GSSG
Guanidoacetic acid
Hexanoylcarnitine (C6)
Hydroxyphenylacetylglycine
Hydroxyproline
Hydroxyproline (HyPro)
Imidazole propionate
Indole-3-lactic acid
Ketodeoxycholic acid
L-Beta-Aspartyl-L-glycine
Methylguanine
Methyluric acid
Monomethylarginine
N-Acetylaspartic acid
o/p/3-Hydroxyphenylacetic acid
Octanoylcarnitine (C8)
Phenyl alanine
Phenylacetyl glutamine
Phenylglyoxylic acid
Pilocarpic acid
Propionylcarnitine (C3)
Pyridoxic acid
Pyrocatechol sulfate
Pyroglutamic acidÂ
Pyroglutamic acid (oxo-proline)
Symmetric dimethylarginine (ADMA)
Trigonelline
N'-Nitrosoanabasine		Alkaloids and derivatives	Alkaloids and derivatives
Pilocarpine		Alkaloids and derivatives	Alkaloids and derivatives
3-Methoxytyramine		Benzenoids	Benzenoids
Benzoic acid	HMDB01870	Benzenoids	Benzene and substituted derivatives
Dihydroxybenzoic acid		Benzenoids	Benzenoids
Gentisuric acid		Benzenoids	Benzenoids
Hippuric acid	HMDB0062583	Benzenoids	Benzene and substituted derivatives
Homogentisic acid	HMDB0000130	Benzenoids	Benzene and substituted derivatives
Methylparaben		Benzenoids	Benzenoids
Phenylacetic acid		Benzenoids	Benzenoids
Propylparaben		Benzenoids	Benzenoids
Salicyluric acid	HMDB00840	Benzenoids	Benzene and substituted derivatives
Syringic acid		Benzenoids	Benzene and substituted derivatives
Vanillic acid	HMDB00484	Benzenoids	Benzene and substituted derivatives
Vanillylmandelic acid	HMDB00291	Benzenoids	Phenols
Phosphate		Homogeneous non-metal compounds	Homogeneous non-metal compounds
Phosphoric acid		Homogeneous non-metal compounds	Homogeneous non-metal compounds
2-Ethylsuberic acid	HMDB0059708	Lipids and lipid-like molecules	Fatty Acyls
2-Hydroxyvaleric acid	HMDB0001970	Lipids and lipid-like molecules	Fatty Acyls
2-Isopropylmalic acid		Lipids and lipid-like molecules	Lipids and lipid-like molecules
2-Methylglutaric acid		Lipids and lipid-like molecules	Lipids and lipid-like molecules
3-Hydroxymethylglutaric acid	HMDB0059737	Lipids and lipid-like molecules	Fatty Acyls
3-Methylglutaconic acid	HMDB0000522	Lipids and lipid-like molecules	Fatty Acyls
Acetylcarnitine	HMDB0000201	Lipids and lipid-like molecules	Fatty Acyls
Azelaic acid	HMDB00784	Lipids and lipid-like molecules	Fatty Acyls
Butyric acid		Lipids and lipid-like molecules	Fatty Acyls
Butyryl-L-carnitine		Lipids and lipid-like molecules	Lipids and lipid-like molecules
Capric acid	HMDB0000513	Lipids and lipid-like molecules	Fatty Acyls
Caprylic acid	HMDB0062511	Lipids and lipid-like molecules	Fatty Acyls
Cholic acid	HMDB0000619	Lipids and lipid-like molecules	Steroids and steroid derivatives
Citraconic acid		Lipids and lipid-like molecules	Lipids and lipid-like molecules
Citramalic acid	HMDB0176421	Lipids and lipid-like molecules	Fatty Acyls
Deoxycarnitine		Lipids and lipid-like molecules	Lipids and lipid-like molecules
Deoxycholic acid	HMDB0000626	Lipids and lipid-like molecules	Steroids and steroid derivatives
Glycerophosphorylcholine		Lipids and lipid-like molecules	Lipids and lipid-like molecules
Isovaleric acid		Lipids and lipid-like molecules	Fatty Acyls
Lauric acid	HMDB0000638	Lipids and lipid-like molecules	Fatty Acyls
Malonyl-L-carnitine		Lipids and lipid-like molecules	Lipids and lipid-like molecules
Methylsuccinic acid	HMDB0029127	Lipids and lipid-like molecules	Fatty Acyls
Pimelic acid		Lipids and lipid-like molecules	Fatty Acyls
Propionyl-L-carnitine		Lipids and lipid-like molecules	Lipids and lipid-like molecules
Suberic acid	HMDB00893	Lipids and lipid-like molecules	Fatty Acyls
Taurocholic Acid	HMDB0000036	Lipids and lipid-like molecules	Steroids and steroid derivatives
Taurodeoxycholic Acid	HMDB0000896	Lipids and lipid-like molecules	Steroids and steroid derivatives
Tiglyl-L-carnitine		Lipids and lipid-like molecules	Lipids and lipid-like molecules
1-Methylguanosine		Nucleosides, nucleotides, and analogues	Nucleosides, nucleotides, and analogues
ADP	HMDB01341	Nucleosides, nucleotides, and analogues	Purine nucleotides
AMP	HMDB00045	Nucleosides, nucleotides, and analogues	Purine nucleotides
ATP	HMDB00538	Nucleosides, nucleotides, and analogues	Purine nucleotides
cAMP		Nucleosides, nucleotides, and analogues	Purine nucleotides
GTP	HMDB01273	Nucleosides, nucleotides, and analogues	Purine nucleotides
IMP	HMDB00175	Nucleosides, nucleotides, and analogues	Purine nucleotides
Methylcytidine		Nucleosides, nucleotides, and analogues	Nucleosides, nucleotides, and analogues
NAD+		Nucleosides, nucleotides, and analogues	Nucleosides, nucleotides, and analogues
NADP+		Nucleosides, nucleotides, and analogues	Nucleosides, nucleotides, and analogues
Pseudouridine		Nucleosides, nucleotides, and analogues	Nucleosides, nucleotides, and analogues
UTP		Nucleosides, nucleotides, and analogues	Pyrimidine nucleotides
2-Aminooctanoic acid	HMDB0000991	Organic acids and derivatives	Carboxylic acids and derivatives
2-Furoylglycine	HMDB00439	Organic acids and derivatives	Carboxylic acids and derivatives
2-Hydroxy-5-oxoproline		Organic acids and derivatives	Organic acids and derivatives
2-Hydroxybutyric acid	HMDB0030097	Organic acids and derivatives	Hydroxy acids and derivatives
2-Methylbutyrylglycine	HMDB0000339	Organic acids and derivatives	Carboxylic acids and derivatives
3-Hydroxybutyric acid	HMDB0000766	Organic acids and derivatives	Hydroxy acids and derivatives
3-Methyl-2-oxovaleric acid	HMDB0000491	Organic acids and derivatives	Keto acids and derivatives
3-Methylhistidine	HMDB0155761	Organic acids and derivatives	Carboxylic acids and derivatives
4-Guanidinobutanoic acid		Organic acids and derivatives	Organic acids and derivatives
5-Aminolevulinic acid	HMDB0001149	Organic acids and derivatives	Carboxylic acids and derivatives
5-Aminopentanoic acid	HMDB0003355	Organic acids and derivatives	Carboxylic acids and derivatives
Acesulfame K		Organic acids and derivatives	Organic acids and derivatives
Acetaminophen sulfate		Organic acids and derivatives	Organic sulfuric acids and derivatives
Acetate	HMDB00042	Organic acids and derivatives	Carboxylic acids and derivatives
Acetoacetic acid	HMDB0000060	Organic acids and derivatives	Keto acids and derivatives
Acetylcarnosine	HMDB0012881	Organic acids and derivatives	Peptidomimetics
Alanine	HMDB0061370	Organic acids and derivatives	Carboxylic acids and derivatives
Alpha-aminoadipic acid		Organic acids and derivatives	Organic acids and derivatives
Alpha-ketoisovaleric acid		Organic acids and derivatives	Organic acids and derivatives
Aminoadipic acid	HMDB0062113	Organic acids and derivatives	Carboxylic acids and derivatives
Arginine	HMDB0003416	Organic acids and derivatives	Carboxylic acids and derivatives
Asparagine	HMDB0000168	Organic acids and derivatives	Carboxylic acids and derivatives
Aspartic acid	HMDB00191	Organic acids and derivatives	Carboxylic acids and derivatives
Betaine	HMDB0000043	Organic acids and derivatives	Carboxylic acids and derivatives
Carnosine	HMDB00033	Organic acids and derivatives	Peptidomimetics
Cis-Aconitic acid		Organic acids and derivatives	Organic acids and derivatives
Citric acid	HMDB0000094	Organic acids and derivatives	Carboxylic acids and derivatives
Citrulline	HMDB0000904	Organic acids and derivatives	Carboxylic acids and derivatives
Creatine	HMDB0000064	Organic acids and derivatives	Carboxylic acids and derivatives
Creatinine	HMDB0000562	Organic acids and derivatives	Carboxylic acids and derivatives
Cystathionine	HMDB00099	Organic acids and derivatives	Carboxylic acids and derivatives
Cysteine	HMDB00574	Organic acids and derivatives	Carboxylic acids and derivatives
Cysteineglutathione disulfide		Organic acids and derivatives	Organic acids and derivatives
Cysteinylglycine		Organic acids and derivatives	Organic acids and derivatives
Cysteinylglycine disulfide	HMDB0000709	Organic acids and derivatives	Carboxylic acids and derivatives
Cystine	HMDB0000192	Organic acids and derivatives	Carboxylic acids and derivatives
Fumaric acid	HMDB00134	Organic acids and derivatives	Carboxylic acids and derivatives
GABA		Organic acids and derivatives	Organic acids and derivatives
Glutaconic acid		Organic acids and derivatives	Organic acids and derivatives
Glutamate	HMDB0060475	Organic acids and derivatives	Carboxylic acids and derivatives
Glutamic acid	HMDB0000148	Organic acids and derivatives	Carboxylic acids and derivatives
Glutamine	HMDB0133927	Organic acids and derivatives	Carboxylic acids and derivatives
Glutaric acid	HMDB0034365	Organic acids and derivatives	Carboxylic acids and derivatives
Glycine	HMDB0000123	Organic acids and derivatives	Carboxylic acids and derivatives
Glycolic acid	HMDB0000115	Organic acids and derivatives	Hydroxy acids and derivatives
Glycylhistidine		Organic acids and derivatives	Organic acids and derivatives
Glycylproline	HMDB0000721	Organic acids and derivatives	Carboxylic acids and derivatives
GSH		Organic acids and derivatives	Organic acids and derivatives
Guanidinosuccinic acid	HMDB0003157	Organic acids and derivatives	Carboxylic acids and derivatives
Hexanoylglycine	HMDB00701	Organic acids and derivatives	Carboxylic acids and derivatives
Histidine	HMDB0154888	Organic acids and derivatives	Carboxylic acids and derivatives
Homoarginine		Organic acids and derivatives	Carboxylic acids and derivatives
Homocitrulline	HMDB00679	Organic acids and derivatives	Carboxylic acids and derivatives
Hydroxyethyl glycine	HMDB0061148	Organic acids and derivatives	Carboxylic acids and derivatives
Hydroxylysine		Organic acids and derivatives	Organic acids and derivatives
Hypotaurine		Organic acids and derivatives	Sulfinic acids and derivatives
Iminodiacetic acid		Organic acids and derivatives	Organic acids and derivatives
Indoleacetyl glutamine		Organic acids and derivatives	Carboxylic acids and derivatives
Indolylacryloylglycine		Organic acids and derivatives	Organic acids and derivatives
indoxyl sulfate	HMDB0186746	Organic acids and derivatives	Organic sulfuric acids and derivatives
Isoleucine	HMDB0000172	Organic acids and derivatives	Carboxylic acids and derivatives
Lactic acid	HMDB0041561	Organic acids and derivatives	Hydroxy acids and derivatives
Lauryl sulfate		Organic acids and derivatives	Organic acids and derivatives
Leucine	HMDB0000687	Organic acids and derivatives	Carboxylic acids and derivatives
Levulinic acid	HMDB0029696	Organic acids and derivatives	Keto acids and derivatives
Lysine	HMDB0000182	Organic acids and derivatives	Carboxylic acids and derivatives
Malic acid	HMDB0167333	Organic acids and derivatives	Hydroxy acids and derivatives
Methionine	HMDB0000696	Organic acids and derivatives	Carboxylic acids and derivatives
Methionine Sulfoxide	HMDB0002005	Organic acids and derivatives	Carboxylic acids and derivatives
N-Acetylputrescine		Organic acids and derivatives	Carboximidic acids and derivatives
N-Butyrylglycine	HMDB0000808	Organic acids and derivatives	Carboxylic acids and derivatives
Nicotinuric acid		Organic acids and derivatives	Organic acids and derivatives
Ornithine	HMDB0062620	Organic acids and derivatives	Carboxylic acids and derivatives
Oxo-proline		Organic acids and derivatives	Organic acids and derivatives
Oxoglutarate	HMDB00208	Organic acids and derivatives	Keto acids and derivatives
Oxoglutaric acid	HMDB0000346	Organic acids and derivatives	Keto acids and derivatives
p-Cresol sulfate	HMDB0133325	Organic acids and derivatives	Organic sulfuric acids and derivatives
Phenyl sulfate		Organic acids and derivatives	Organic acids and derivatives
Phenylacetylglutamine	HMDB0006344	Organic acids and derivatives	Carboxylic acids and derivatives
Phenylacetylglycine	HMDB0000821	Organic acids and derivatives	Carboxylic acids and derivatives
Phenylalanine	HMDB0159744	Organic acids and derivatives	Carboxylic acids and derivatives
Phenylpropionylglycine		Organic acids and derivatives	Organic acids and derivatives
Phenylsulfate		Organic acids and derivatives	Organic sulfuric acids and derivatives
Phosphocreatine		Organic acids and derivatives	Carboxylic acids and derivatives
Pipecolate	HMDB00070	Organic acids and derivatives	Carboxylic acids and derivatives
Pipecolic acid	HMDB0062767	Organic acids and derivatives	Carboxylic acids and derivatives
Proline	HMDB0000162	Organic acids and derivatives	Carboxylic acids and derivatives
Proline betaine	HMDB0004827	Organic acids and derivatives	Carboxylic acids and derivatives
Prolylglycine	HMDB0011178	Organic acids and derivatives	Carboxylic acids and derivatives
Propenoyl-L-carnitine		Organic acids and derivatives	Organic acids and derivatives
Propionate	HMDB00237	Organic acids and derivatives	Carboxylic acids and derivatives
Propionylglycine	HMDB0000783	Organic acids and derivatives	Carboxylic acids and derivatives
Pyridylacetylglycine		Organic acids and derivatives	Organic acids and derivatives
Pyroglutamic acid	HMDB0000267	Organic acids and derivatives	Carboxylic acids and derivatives
Pyruvic acid	HMDB0141776	Organic acids and derivatives	Keto acids and derivatives
Serine	HMDB0001264	Organic acids and derivatives	Carboxylic acids and derivatives
Succinic acid	HMDB0000254	Organic acids and derivatives	Carboxylic acids and derivatives
Succinylacetone	HMDB00635	Organic acids and derivatives	Keto acids and derivatives
Taurine	HMDB0000251	Organic acids and derivatives	Organic sulfonic acids and derivatives
Theanine	HMDB0062764	Organic acids and derivatives	Carboxylic acids and derivatives
Threonine	HMDB0000167	Organic acids and derivatives	Carboxylic acids and derivatives
Tiglylglycine	HMDB0002393	Organic acids and derivatives	Carboxylic acids and derivatives
Trimethyllysine		Organic acids and derivatives	Organic acids and derivatives
Tryptophan betaine		Organic acids and derivatives	Organic acids and derivatives
Tyrosine	HMDB0000158	Organic acids and derivatives	Carboxylic acids and derivatives
Urea	HMDB00294	Organic acids and derivatives	Organic carbonic acids and derivatives
Valine	HMDB0000883	Organic acids and derivatives	Carboxylic acids and derivatives
Valylvaline		Organic acids and derivatives	Organic acids and derivatives
1-Phenylethylamine	HMDB0002017	Organic nitrogen compounds	Organonitrogen compounds
Acetylcholine	HMDB0000895	Organic nitrogen compounds	Organonitrogen compounds
Cadaverine	HMDB02322	Organic nitrogen compounds	Organonitrogen compounds
Carnitine	HMDB0062496	Organic nitrogen compounds	Organonitrogen compounds
Choline	HMDB0000097	Organic nitrogen compounds	Organonitrogen compounds
Phosphorylcholine	HMDB0001565	Organic nitrogen compounds	Organonitrogen compounds
Putrescine	HMDB01414	Organic nitrogen compounds	Organonitrogen compounds
Spermidine		Organic nitrogen compounds	Organonitrogen compounds
Spermine		Organic nitrogen compounds	Organonitrogen compounds
Trimethylamine N-oxide	HMDB0000925	Organic nitrogen compounds	Organonitrogen compounds
2,3-Dehydro-2-deoxy-N-acetylneuraminic acid		Organic oxygen compounds	Organic oxygen compounds
Acetaminophen glucuronide		Organic oxygen compounds	Organooxygen compounds
Acetylneuraminic acid		Organic oxygen compounds	Organic oxygen compounds
CTP		Organic oxygen compounds	Organooxygen compounds
Ethyl glucuronide		Organic oxygen compounds	Organooxygen compounds
Glucaric acid		Organic oxygen compounds	Organooxygen compounds
Gluconic acid		Organic oxygen compounds	Organooxygen compounds
Glucose	HMDB0000516	Organic oxygen compounds	Organooxygen compounds
Glucose-6-phosphate (G6P)		Organic oxygen compounds	Organooxygen compounds
Glyceraldehyde 3-phosphate		Organic oxygen compounds	Organooxygen compounds
Kynurenine	HMDB0000684	Organic oxygen compounds	Organooxygen compounds
N-Acetyl-9-O-acetylneuraminic acid		Organic oxygen compounds	Organic oxygen compounds
N-acetylneuraminic acid	HMDB0000230	Organic oxygen compounds	Organooxygen compounds
N-Glycolylneuraminic acid		Organic oxygen compounds	Organic oxygen compounds
p-Cresol glucuronide		Organic oxygen compounds	Organic oxygen compounds
Pantothenic acid	HMDB0062717	Organic oxygen compounds	Organooxygen compounds
Quinic acid	HMDB0172071	Organic oxygen compounds	Organooxygen compounds
Tartaric acid	HMDB0000346	Organic oxygen compounds	Organooxygen compounds
Threonic acid	HMDB0137260	Organic oxygen compounds	Organooxygen compounds
1-Methylnicotinamide	HMDB0000699	Organoheterocyclic compounds	Pyridines and derivatives
1-Methyluric acid	HMDB0001882	Organoheterocyclic compounds	Imidazopyrimidines
1,3-Dimethyluric acid	HMDB0030801	Organoheterocyclic compounds	Imidazopyrimidines
3-Methyladenine		Organoheterocyclic compounds	Organoheterocyclic compounds
5-Acetylamino-6-formylamino-3-methyluracil		Organoheterocyclic compounds	Organoheterocyclic compounds
7-Methylxanthine	HMDB0001991	Organoheterocyclic compounds	Imidazopyrimidines
Adenine	HMDB00034	Organoheterocyclic compounds	Imidazopyrimidines
Ascorbic acid	HMDB0000044	Organoheterocyclic compounds	Dihydrofurans
Caffeine	HMDB0001847	Organoheterocyclic compounds	Imidazopyrimidines
Dihydrothymine		Organoheterocyclic compounds	Organoheterocyclic compounds
Guanine		Organoheterocyclic compounds	Imidazopyrimidines
Hydroxycotinine		Organoheterocyclic compounds	Organoheterocyclic compounds
Hypoxanthine	HMDB0000157	Organoheterocyclic compounds	Imidazopyrimidines
Indole-3-acetamide		Organoheterocyclic compounds	Organoheterocyclic compounds
Indole-3-carboxaldehyde		Organoheterocyclic compounds	Organoheterocyclic compounds
Indoleacetic acid	HMDB0000197	Organoheterocyclic compounds	Indoles and derivatives
Kynurenic Acid	HMDB00715	Organoheterocyclic compounds	Quinolines and derivatives
Orotic acid		Organoheterocyclic compounds	Diazines
Oxypurinol	HMDB0000786	Organoheterocyclic compounds	Imidazopyrimidines
Quinolinic acid	HMDB00232	Organoheterocyclic compounds	Pyridines and derivatives
Saccharin		Organoheterocyclic compounds	Organoheterocyclic compounds
Thymine	HMDB0010337	Organoheterocyclic compounds	Diazines
Trans-urocanate		Organoheterocyclic compounds	Organoheterocyclic compounds
Trans-urocanic acid		Organoheterocyclic compounds	Organoheterocyclic compounds
Tryptophan	HMDB0030396	Organoheterocyclic compounds	Indoles and derivatives
Uracil	HMDB0000300	Organoheterocyclic compounds	Diazines
Uric acid	HMDB0000289	Organoheterocyclic compounds	Imidazopyrimidines
Urocanic acid	HMDB0062562	Organoheterocyclic compounds	Azoles
Xanthine	HMDB0000292	Organoheterocyclic compounds	Imidazopyrimidines
Cinnamic acid		Phenylpropanoids and polyketides	Phenylpropanoids and polyketides
Ferulic acid	HMDB0000954	Phenylpropanoids and polyketides	Cinnamic acids and derivatives